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polyclonal anti rab11a  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc polyclonal anti rab11a
    Polyclonal Anti Rab11a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+anti+rab11a/pmc12326875-75-104-108?v=Cell+Signaling+Technology+Inc
    Average 86 stars, based on 1 article reviews
    polyclonal anti rab11a - by Bioz Stars, 2026-06
    86/100 stars

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    ( A ) Distribution of endocytic vesicles in either untreated IE cultures or upon 24h exposure to HIV-1 Gag-iGFP by confocal microscopy analyses. Endocytic vesicle markers (red); Early endosomes (EEA1, Early endosome antigen 1), Autophagosomes (LC3, Microtubule-associated protein 1A/1B-light chain 3), Recycling endosomes <t>(Rab11a,</t> Ras-related protein Rab11a), Multivesicular late endosomes (LBPA, lysobisphosphatidic acid), and nuclei (blue). Scale bar = 20 micron, representative of n = 3 donors. ( B ) Colocalization of endocytic vesicle markers in IE cultures upon 24h exposure to HIV-1 Gag-iGFP by confocal microscopy analyses. Endocytic vesicle markers (EEA1, LC3, Rab11a, LBPA, red), HIV-1 (green, filled arrowheads). Scale bar = 20 micron, representative of n = 3 donors. (C) Histograms of EEA1, LC3, Rab11a or LBPA and HIV-1 fluorescence intensities depicting the degree of overlap between HIV-1 and respective endocytic vesicle along the indicated 6 micron-long Region of Interest (ROI, as specified in B). ( D ) Pearson’s correlation coefficient for colocalization analyses between each endocytic vesicle marker and HIV-1, n = 3 donors, Two-way ANOVA, **** p <0.0001.
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    ( A ) Distribution of endocytic vesicles in either untreated IE cultures or upon 24h exposure to HIV-1 Gag-iGFP by confocal microscopy analyses. Endocytic vesicle markers (red); Early endosomes (EEA1, Early endosome antigen 1), Autophagosomes (LC3, Microtubule-associated protein 1A/1B-light chain 3), Recycling endosomes (Rab11a, Ras-related protein Rab11a), Multivesicular late endosomes (LBPA, lysobisphosphatidic acid), and nuclei (blue). Scale bar = 20 micron, representative of n = 3 donors. ( B ) Colocalization of endocytic vesicle markers in IE cultures upon 24h exposure to HIV-1 Gag-iGFP by confocal microscopy analyses. Endocytic vesicle markers (EEA1, LC3, Rab11a, LBPA, red), HIV-1 (green, filled arrowheads). Scale bar = 20 micron, representative of n = 3 donors. (C) Histograms of EEA1, LC3, Rab11a or LBPA and HIV-1 fluorescence intensities depicting the degree of overlap between HIV-1 and respective endocytic vesicle along the indicated 6 micron-long Region of Interest (ROI, as specified in B). ( D ) Pearson’s correlation coefficient for colocalization analyses between each endocytic vesicle marker and HIV-1, n = 3 donors, Two-way ANOVA, **** p <0.0001.

    Journal: PLOS Pathogens

    Article Title: HIV-1 exploits LBPA-dependent intraepithelial trafficking for productive infection of human intestinal mucosa

    doi: 10.1371/journal.ppat.1012714

    Figure Lengend Snippet: ( A ) Distribution of endocytic vesicles in either untreated IE cultures or upon 24h exposure to HIV-1 Gag-iGFP by confocal microscopy analyses. Endocytic vesicle markers (red); Early endosomes (EEA1, Early endosome antigen 1), Autophagosomes (LC3, Microtubule-associated protein 1A/1B-light chain 3), Recycling endosomes (Rab11a, Ras-related protein Rab11a), Multivesicular late endosomes (LBPA, lysobisphosphatidic acid), and nuclei (blue). Scale bar = 20 micron, representative of n = 3 donors. ( B ) Colocalization of endocytic vesicle markers in IE cultures upon 24h exposure to HIV-1 Gag-iGFP by confocal microscopy analyses. Endocytic vesicle markers (EEA1, LC3, Rab11a, LBPA, red), HIV-1 (green, filled arrowheads). Scale bar = 20 micron, representative of n = 3 donors. (C) Histograms of EEA1, LC3, Rab11a or LBPA and HIV-1 fluorescence intensities depicting the degree of overlap between HIV-1 and respective endocytic vesicle along the indicated 6 micron-long Region of Interest (ROI, as specified in B). ( D ) Pearson’s correlation coefficient for colocalization analyses between each endocytic vesicle marker and HIV-1, n = 3 donors, Two-way ANOVA, **** p <0.0001.

    Article Snippet: Primary antibodies included mouse IgG1 anti-p24 (clone KC57, Beckman Coulter), mouse IgG2b anti-CD11c (clone S-HCL-3, BD bioscience), mouse IgG1 anti-LC3 (clone 4E12, MBL), rat IgG2a anti-ZO-1 (clone R40.76, Santa Cruz biotechnology), mouse IgG2b anti-E-Cad (clone 36/E-Cadherin (RUO, BD Bioscience), mouse IgG1 anti-LBPA (clone 6C4, Sigma Aldrich), polyclonal rabbit anti-Rab11a (71–5300, Invitrogen), mouse IgG1 anti-EEA1 (clone 14/EEA1 (RUO), BD bioscience), mouse IgG1 anti-CD63 (clone MEM-259, Invitrogen) and mouse IgG2a anti-GFP (clone B2, Santa Cruz Biotechnology).

    Techniques: Confocal Microscopy, Fluorescence, Marker